Nutra-pharmaceutical potential evaluation of Trignollafoenum graecum seeds extracts in different solvents

Syeda Mona Hassan and Momina Arshad

Abstract

This research work has been designed to evaluate the antioxidant and antimicrobial potential of Trigonellafoenum graecum seeds. The phytochemical, antifungal and antioxidant components of Trigonellafoenum graecum seed was extracted by using four solvent systems (80% methanol, 80% ethanol, 100% methanol and 100% ethanol). Trigonellafoenum graecum seeds showed maximum extract yield (42.9 g/100g DW) in 80% methanolic solvent system. Antioxidant analysis of Trigonellafoenum graecum seeds extracts was performed in terms of total phenolic and total flavonoid contents, showed that 80% methanolic extract presented highest total phenolic contents (70.2 mg GAE/g DW), Also 80% methanolic extract showed maximum total flavonoid contents (93.2 mg CE/g DW). Antioxidant activity was determined by DPPH radical scavenging activity and measure of reducing power. Results revealed that 80% methanolic extract showed highest radical scavenging activity and reducing potential. Antimicrobial activity of Trigonellafoenum graecum seeds was investigated by Disc Diffusion Method. Results showed that 80% methanolic extract exhibited highest antibacterial and antifungal potential against E. coli (30 mm DIZ) and A. Paraciticus (29 mm DIZ), respectively. Overall, the present investigation showed that 80% methanolic Trigonellafoenum graecum seeds extract can be used effectively to make antimicrobial, antioxidant agents which can be utilized in different nutra-pharmaceutical industries.