Green synthesis and characterization of copper nanoparticles using Parthenium hysterophorus extract: Antibacterial and antioxidant activities evaluation

Arif Nazir, Shafiq ur Rehman, Mazhar Abbas, Dure N. Iqbal, Ahmad R. Ahsraf and Munawar Iqbal

Abstract

In view of green route simplicity, cost effectiveness and eco-benign approach, this study focusses on the fabrication and characterization of copper nanoparticles (Cu NPs) using leaf extract (aqueous) of Parthenium hysterophorus (Gajar Booti). Characterization of P. hysterophorus mediated Cu NPs was performed by UV-Visible, Fourier-transform infrared, X-ray powder diffraction and scanning electron microscopy techniques. The UV-Vis characteristic peak was observed at 563 nm along with change in variation in color from light brown to dark green confirm the formation of Cu NPs. The Cu NPs was semi-spherical with average particle size of 15.48 nm. These green mediated Cu NPs was analyzed for their antibacterial activity by disc diffusion and MIC analysis and antibacterial activity was promising against Escherichia coli, Pasteurella multocida, Staphylococcus aureus and Bacillus subtilis strains. The Cu NPs also showed excellent antioxidant activity evaluated by DPPH free radical scavenging. In view of promising bioactivity, the Cu NPs prepared using P. hysterophorus extract could be used for biomedical applications.