Benzocaine derivative determination via furan-2-carboxaldehyde reagent using differential pulse polarographic method

Raied H. Mohammad, Taghreed M. Musa and Ameerah H. Atiyah

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Benzocaine (BE) has been determined by the indirect differential pulse polarographic method (DPP). The suggested method is based on the synthesis of benzocaine via furan -2-carboxaldehyde reagent in presence of hydrochloric acid, then the diazotized at 0-5 oC with sodium nitrite to obtain a new benzocaine derivative. Also finding the values half-wave potential (E½) and electrochemical behavior using the dropping mercury electrode (DME). Optimum conditions affecting a quantitative analysis of benzocaine and its derivative such as solvent, pH, buffer solution and supporting electrolyte effect were explored. The benzocaine derivative showed a clear reduction peak at a potential of (-0.93 V) under optimal conditions. The standard calibration curve prepared for the benzocaine derivative at a concentration range of (5-100) µ gave a correlation coefficient of 0.9998, also limit of detection (LOD) and limit of quantitation (LOQ) of the benzocaine drug was 20.096 and 6.949 µ respectively, where the average relative standard deviation RSD of the benzocaine derivative was 0.41 % with recovery 100.40% (n=5). Based on the observation, the developed method could be employed for the estimation of benzocaine in pharmaceutical formulations.