Validated UV-Visible spectrometry using water as a solvent for determination of chloroquine in tablet samples

Kidanemariam Desta and Meareg Amare

Abstract

Methods reported including the official methods for determination of chloroquine in tablet samples use carcinogenic organic solvents. In this study, UV-Vis spectrometry using water as a solvent was developed for determination of chloroquine phosphate in pharmaceutical tablet dosage forms. The method was validated according to the International Conference on Harmonization (ICH) and USP guidelines. The absorbance of chloroquine phosphate in distilled water at  max of 343 nm showed linear dependence on concentration in the range 10.88-30.56 µg mL-1 with determination coefficient of 0.99972. Recovery results in the range 98.79–101.20% and low coefficient of variation values for intra-day and interday precisions (0.37% and 0.76%, respectively) showed the accuracy and reproducibility of the method. The method was used for determination of chloroquine phosphate in tablet formulations of different brands. Results in the range 100.63–103.52% of the labeled chloroquine phosphate in tablet formulations confirmed the applicability of the developed method for real sample analysis. Hence, the developed UV-Vis method using environmentally friendly water as a solvent, with an excellent accuracy and precision showed that the developed method can be a potential substituent for the official reference methods.