Issue 6

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Ladokun Olusola and Arojojoye Oluwatosin. 2016. Extraction of polyphenol oxidase from green and red apple fruits and the effect of pH variation on the activity of the enzyme. Current Science Perspectives 2(2) 14-18

Abstract

Polyphenol oxidase is present in the majority of plant tissues and it is involved in adverse browning of plant products. Crude polyphenol oxidase (PPO) was extracted from flesh and peels of green and red apple fruits and the concentration of the enzyme in the flesh and peels of the fruits was determined spectrophotometrically. The activity of the enzyme in the green apple peel, green apple flesh, red apple peel and red apple flesh were found to be 2.15, 1.30, 0.70 and 2.65 µmolmin-1cm-3, respectively. The effect of pH on the stability of the enzyme’s activity was also investigated. Catechol at a concentration of 20 mM was used as a substrate while sodium acetate buffer (0.2M) with a pH range of 3.6-5.6 and sodium phosphate buffer (0.2M) with a pH range of 6.0-8.0 were used to monitor the effect of pH on the activity of the enzyme. From the results obtained, optimum pH for crude PPO extracted from the green apple peel, green apple flesh, red apple peel and red apple flesh were 7.6, 6.4, 8.4 and 8.0 respectively. Increase or decrease of pH from this range caused decrease in the activity of the enzyme. This can therefore be a good way of controlling undesirable changes in these fruits.

 

1.  Mohammad Asif. 2016. A review on antiepileptic drug and their uses, mechanism of actions, adverse effects and drug interaction. Current Science Perspectives 2(2) 19-38

Abstract

The main objective of antiepileptic drug (AED) therapy is to permit patients to maintain a normal lifestyle by totally control of seizures with minimal adverse effects. Phenobarbital (PBT), the first extensively used AED, consequently surge in AEDs such as valproic acid (VLPA), bezodiazepines (BZDs) and phenytoin (PHT) was a direct importance of the progress of animal seizure models. Thus many AEDs are developed and associated with dose limiting adverse effects, adverse reactions and toxicity by drug-drug interactions. The awareness that these early compounds could be further optimized for acceptability and properties has rational drug design efforts for progress of subsequent AEDs. Normally AEDs modulate voltage-gated ion channels, facilitate inhibitory neurotransmissions, reduce excitatory neurotransmissions and/or adjust synaptic release. This information, coupled with genetic links with epilepsy, has assisted a more recent target-based approach to novel AEDs.

 

 

Brijesh Kumar Pandey, Savita Vyas, Mukesh Pandey and Anurag Gaur. 2016. Municipal solid waste to energy conversion methodology as physical, thermal, and biological methods. Current Science Perspectives 2(2) 39-44

Abstract

This paper presents the study of municipal solid waste as a potential source of renewable energy. Municipal solid waste management is one of major problems not only in India but also in whole world. The Study reveals that main component of municipal solid waste (MSW) comprises the biomass material such as food, paper, wood waste, clothes rage, rubber, plastics and other daily used discarded materials. Various studies show that about 90% municipal solid waste is disposed of unscientifically and unmannered way in open dump places which create problems to public health, the environmental problems and distort the surround aesthetic beauty. In this paper an attempt has been made to provide an exclusive way of power generation methodology that derives power from municipal solid waste. This paper suggests an ideology for effective power generation from municipal solid waste and variation of different technology from their viability and effectiveness.