New validated method for analysis of salymarin in polyherbal formulation (aqueous extract, oral liquid and solid dosage form)

Somia Gul, Kashifa Khanum and Nusrat Mujtaba

Abstract

A UV/visible Spectrophotometric method for the determination of Silymarin in poly herbal formulation have been developed. Developed method is based on the simple concept of solubility of chemical i.e. Silymarin in methanol. This method is
simple, rapid, accurate, sensitive and precise for the determination of Silymarin in polyherbal formulation like aqueous extract, Oral liquid (i.e. Syrup) and solid (i.e. Capsules) dosage form containing crude herb Silybum marianum. The ?max of
Silymarin measured at 287 nm. The molecule (Silymarin) obeys Beer’s law in between range of 0.3–1.6 mg/ml. The method is reproducible, accurate and linear for routine quality control testing of phyto-pharmaceutical dosage forms.