Physicochemical profiling of crinum zeylanicum oil: Antifungal activity of its bulb extracts in n-hexane, ethanol and water
Janet Moses Daben, Dayil Albert Dashak and Amina Mika Lohdip
Abstract
This study was performed to determine the quality and medicinal values of the oil and the anti-fungal activity of the extracts to buttress traditional medicinal practices. Soxhlet extraction of n-hexane, ethanol and water extracts were performed and antifungal activity was evaluated against Yeast, Aspergillus niger, Aspergillus fumigates and Aspergillus flavus. The oil percentage (3.71±1.03%), moisture content (57.65±1.16%), and refractive index (1.4667±0.06). Saponification value (205.62±0.58 mg/KOH/g), peroxide value (4.59±1.12 meq/kg), iodine value (129.74±0.39 mg/g), free fatty acid (5.93±1.02%), acid value (11.80±1.72 mg/KOH/kg), relative density at 250C (0.86kg/m3), viscosity (0.94±0.93) was recorded, while soap content was not detected. The antifungal susceptible test of the water extract showed a marked inhibitory effect on all the test organisms especially on A. niger more than the control at 300 mg/mL, (p < 0.0001); having the least inhibitory activity on yeast. N-hexane and ethanol extracts have a significant effect on A. niger and the least on A. fumigatus. The Minimum inhibitory concentration (MIC) revealed n-hexane extract at 75 mg/mL having a potent effect on Yeast, A. flavus, A. fumigates and for water and ethanol extracts varied at 37.5 mg/mL to 150 mg/mL on A. flavus respectively. The minimum fungicidal concentration (MFC) of water extract remarkably showed resistance on A. flavus and the least action on yeast ranging from 7.5 to 150 (mg/mL). This study indicated that the oil has low susceptibility and potent anti-fungal activity suitable for health benefits.
